From NuGOwiki
Lipgene metabolomics standard operation procedure for blood and urine collection
From the moment of sample collection and storage, temperature can influence sample stability, therefore it is vital that temperature recommendations for metabolomics sampling are adhered to. In addition, selection of blood collection tubes is important because anticoagulants and preservatives in tubes can affect the quality of biomarkers. The following amendments to the SOP are directed towards protocols for blood and urine collection:
Blood Collection (amendment to Section 4.4.1)
An additional fasting plasma sample will be collected at the -5 minutes time point during the IVGTT, both pre and post clinical intervention:
Vacutainer: 4ml glass lithium heparin tube (Green). Collection: Follow instructions in current SOP. Keep the tube on ice prior to collection and up until freezing. Processing: Centrifuge using the current recommendations. Remove plasma in three 500μl aliquots (two to be sent for central analysis, the other to be kept as a duplicate sample at the centre in which it was collected). Labelling: Use the spare blank labels that are provided with the other LIPGENE labels and record the following: Subject number: XXXXX Analyte name: Metabolomics Plasma Sampling time: Lipgene 1 / 2 Storage: Metabolomics plasma samples should be stored in their own individual boxes: O and OO. O will be sent for central analysis and OO will be kept at the centre in which the samples are collected. Follow the directions in the “box inlays” section on the LIPGENE website under WP 1.2 SOPs (in internal reports), for arrangement of the samples in the boxes. Store at -80˚C and transport frozen to central analysis centre at TCD along with the other phase 2 samples in September 2006.
Urine Collection (amendment to Section 4.5)
No additional urine sample will be collected. The current collection method will be adjusted to ensure the sample is chilled (4-8˚C) from the moment of collection until it is processed and frozen.
Collection: Originally all subjects were provided with a vessel to collect their first void urine on the morning of the IVGTT and this would be given to the investigators on arrival at the centre. Centres wishing to use urine for metabolomics analysis must ensure that samples are collected in a chilled container and are kept chilled until arrival at the centre. Therefore, subjects must be supplied with a small pack which contains the urine collection vessel along with either ice capsules or freezer sachets. This pack must be placed in the subject’s home freezer so that it is ready to be used on the morning of the IVGTT (pre and post clinical intervention).